Journal: iScience
Article Title: Non-random spatial organization of telomeres varies during the cell cycle and requires LAP2 and BAF
doi: 10.1016/j.isci.2024.109343
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Article Snippet: The list of antibodies used can be found in the and the combination of primary & secondary antibodies used were: 1:100 GFP-nanobody conjugated with Atto488 (NanoTag) for TRF1-GFP, 1:1000 mouse anti-lamin A/C (Santa Cruz) and horse anti-mouse Dylight549 or donkey anti-mouse Dylight 550, 1:350 rabbit anti-SUN1 (Sigma) and/or rabbit anti-CEP152 (Sigma) and goat anti-rabbit Alexa-647; 1:1000 mouse anti-lamin A/C (Santa Cruz) and horse anti-mouse Dylight488, 1:2000 human anti-CREST (Sigma) and donkey anti-human Dylight550, 1:350 rabbit anti-SUN1 and goat anti-rabbit Alexa-647; 1:100 GFP-nanobody for TRF1-GFP, 1:250 mouse anti-LAP2α and anti-mouse Dylight549, 1:250 rabbit anti-LAP2β and anti-rabbit Alexa-647.
Techniques: Plasmid Preparation, Recombinant, Saline, Generated, Software